Journal: The Journal of Nutrition

Article Title: Maternal Adiposity and Inflammation: Risk Factors for Iron Deficiency in Pregnancy

doi: 10.1016/j.tjnut.2025.08.022

Figure Lengend Snippet: Changes in mean iron markers throughout pregnancy according to BMI group. Line graphs showing mean and SE. Normal weight: BMI 18.5–24.9 kg/m 2 , overweight: BMI 25.0–29.9 kg/m 2 , obesity: BMI ≥30 kg/m 2 . Repeated measures ANCOVA with Tukey's post-hoc test used to compare differences between timepoints, and general linear model applied for comparisons at each timepoint, adjusting for maternal age, smoking, educational status, and vitamin D intervention group. Special characters denote statistical difference between BMI groups at each timepoint. Special characters next to P values denote temporal differences between BMI categories: ∗normal weight vs. obesity. † normal weight vs. overweight. ‡ overweight vs. obesity. P values at the top of the graph show overall significance between timepoints. ANCOVA, analysis of covariance; GW, gestational weeks; sTfR, soluble transferrin receptor; TIBC, total iron binding capacity; TSAT, transferrin saturation; UIBC, unsaturated iron binding capacity.

Article Snippet: In addition, ID was defined when sTfR was >1.513 μg/mL (97.5 th percentile as reported from Human sTfR ELISA BioVendor Group).

Techniques: Binding Assay

Journal: The Journal of Nutrition

Article Title: Maternal Adiposity and Inflammation: Risk Factors for Iron Deficiency in Pregnancy

doi: 10.1016/j.tjnut.2025.08.022

Figure Lengend Snippet: Accuracy of iron markers at 12 GW for the prediction of iron deficiency at 36 GW defined by sTfR. Receiver operating characteristic (ROC) analysis: (A) all samples. (B) Normal weight (BMI 18.5–24.9 kg/m 2 ). (C) Overweight/obesity (BMI ≥ 25 kg/m 2 ). Iron deficiency at 36 GW defined as sTfR>1.513 μg/mL. P < 0.05 considered significant. GW, gestational weeks; MCH, mean cell hemoglobin; MCHC, mean hemoglobin concentration; MCV, mean cell volume; RDW, red cell distribution width; sTfR, soluble transferrin receptor; TSAT, transferrin saturation.

Article Snippet: In addition, ID was defined when sTfR was >1.513 μg/mL (97.5 th percentile as reported from Human sTfR ELISA BioVendor Group).

Techniques: Concentration Assay

Journal: The British Journal of Nutrition

Article Title: Effect of supplementation with vitamin D on biochemical markers of iron status and erythropoiesis in older people: BEST-D trial

doi: 10.1017/S0007114525103516

Figure Lengend Snippet: Median levels of parameters measured at baseline to define Fe deficiency in healthy male and female participants in the BEST-D study. Box and whisker plots show the median of each parameter with individual outliers shown above and below the inter-quintile range for (a) Fe, (b) transferrin (Tf), (c) transferrin saturation (TSAT%), (d) Hepcidin and (e) correlation of hepcidin with TSAT% (below dashed line may be deficient in Fe).

Article Snippet: Commercially available ELISA were used to measure bioactive hepcidin (DRG product EIA5782, Lot 314K038) and sTfR (Biovendor product RD194011100, Lot E17046 ).

Techniques: Whisker Assay

Journal: The British Journal of Nutrition

Article Title: Effect of supplementation with vitamin D on biochemical markers of iron status and erythropoiesis in older people: BEST-D trial

doi: 10.1017/S0007114525103516

Figure Lengend Snippet: Measurements of Fe availability for erythropoiesis at baseline and their relationship with hepcidin levels in the BEST-D cohort of healthy male and female participants. (a) sTfR, (b) ferritin, (c) sTfR-ferritin index (sTfR-F index) is shown at baseline before randomisation. Horizontal bars of box and whisker plots represent medians with individual outliers above and below the inter-quintile range. (d) Correlation of hepcidin with sTfR-F index (above dashed line may have reduced erythropoiesis).

Article Snippet: Commercially available ELISA were used to measure bioactive hepcidin (DRG product EIA5782, Lot 314K038) and sTfR (Biovendor product RD194011100, Lot E17046 ).

Techniques: Whisker Assay

Journal: The British Journal of Nutrition

Article Title: Effect of supplementation with vitamin D on biochemical markers of iron status and erythropoiesis in older people: BEST-D trial

doi: 10.1017/S0007114525103516

Figure Lengend Snippet: Measurements of Fe availability for erythropoiesis at baseline and their relationship with hepcidin levels in the BEST-D cohort of healthy male and female participants. (a) sTfR, (b) ferritin, (c) sTfR-ferritin index (sTfR-F index) is shown at baseline before randomisation. Horizontal bars of box and whisker plots represent medians with individual outliers above and below the inter-quintile range. (d) Correlation of hepcidin with sTfR-F index (above dashed line may have reduced erythropoiesis).

Article Snippet: Commercially available ELISA were used to measure bioactive hepcidin (DRG product EIA5782, Lot 314K038) and sTfR (Biovendor product RD194011100, Lot E17046 ).

Techniques: Whisker Assay